Water & Environment

An efficient method to determine the biomass of spirulina cultures with the Photopette. The wavelength of 565 nm is used to determine the biomass concentration, 680 nm to measure the amount of chlorophyll a absorption in the Spirulina. This amount of absorbed light is proportional to the amount of Spirulina present. 750 nm is used to measure the apparent turbidity of the Spirulina.

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Determining Turbidity of an aqueous solution is a frequently used procedure to estimate the quality of water, or even concentration of suspended particles such as cells, bacteria and mud. Following the USEPA method and ISO 7027 method, the turbidity is measured at wavelengths of 570 nm and 850 nm respectively. This application note highlights the measurements of OD at these two wavelengths using the Photopette®.

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