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The food & beverage industry must make many tests and take certain measurements to ensure that their products meet high standards and are safe for consumption. The Photopette can be used for photometric measurements of liquid samples.
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The objective of this application note is to demonstrate how Photopette® Cell can be used to quantify the amount of ethanol in beverages on the example of wine. Alcohol dehydrogenase (ADH) is an enzyme that catalyzes the oxidation of ethanol to acetaldehyde. At the same time, an equimolar amount of nicotinamide adenine dinucleotide (NAD+) is reduced to NADH. The amount of NADH produced is directly proportional to the alcohol concentration in the sample [1]. The produced NADH is measured by its absorbance at 340 nm.
Dal contains proteins and dal with high protein content is preferred. A method to access the dal quality in the field, when purchasing dal from the farmer is needed. The dal protein is extracted and the protein concentrations is directly measuring at 280 nm using Photopette® Bio. The method can be carried out in the field