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Life Science

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UV/VIS spectrophotometry is a common method used for research in most life science laboratories around the globe.

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Here we demonstrate how Photopette® can be used within the cell culture hood to speed up COVID-19 related cell culture research. Determine cell densities, protein and DNA/RNA concentrations within seconds without any sample transfer and perform cell viability studies.

Direct DNA concentration measurement and DNA purity for unknown samples. Furthermore, this application note also acts as a handy guide to get you started with Photopette and outlines application-specific parameters for reference.

The objective of this application note is to demonstrate how Photopette Bio can be used for direct DNA concentration measurement and DNA purity for unknown samples. Furthermore, it acts as a handy guide to get you started with Photopette, and outlines application-specific parameters for reference.

Measurement of the protein concentration using Bradford assay is based on a shift in absorption spectrum of the Coomassie Brilliant Blue G-250 dye. In acidic conditions, protein binds with the dye and alters its absorption maxima from 465 nm to 595 nm Thus, a change in absorbance at 595 nm can be used to quantify protein concentration.
This application note provides an easy and fast enzymatic assay to quantify lactate in biological samples such as serum, plasma, urine, cell culture/fermentation media or in food & beverage products using Photopette®.
Here we demonstrate a method to measure the pantoprazole content in tablets within 10 minutes. There are no reagents necessary. By using the Photopette® handheld spectrophotometer [1, 2] only minimal training is requited. The method is easy and fast performed in two steps 1) Calibration and 2) Measurement. The method can be performed at any location and does not require a lab.
Cell-viability assays are important cell-based assays to determine whether cells are alive or dead. Such assays are widely used for investigating cell response to a given agent or drug or to establish relative cytotoxicity of chemicals. The cell-viability is typically measured by determining innate metabolic activity using redox indicators.
Yeast cell counting at OD600. Almost no training is requited. The method is easy and fast performed at the brewery or in the cell culture hood. Yeast can be directly measured at 600 nm in the cell culture flask. The method can be performed at any location and does not require a lab.
E.coli cell counting at OD600. Almost no training is requited. The method is easy and fast performed at the bioreactor or in the cell culture hood. E.coli can be directly measured at 600 nm in the cell culture flask. The method can be performed at any location and does not require a lab.